DNA fragmentation is a feature of cystic fibrosis epithelial cells: a disease with inappropriate apoptosis?

FEBS Letters

Coletta, Salvatore¹  Auricchio, Salvatore¹  De Marco, Giulio¹  de Ritis, Giorgio¹  Maiuri, Luigi²  Raia, Valeria¹  Londei, Marco³ 

[1]Department of Pediatrics, University Federico II of Naples, via S. Pansini 5, 80131 Naples, Italy

[2]Children's Hospital Pausilipon, Naples, Italy

[3]Kennedy Institute of Rheumatology, Sunley Division/Immunology, London, UK

关键词: Cystic fibrosis;    Cystic fibrosis transmembrane conductance regulator;    DNA fragmentation;    Apoptosis;    Programmed cell death;    CF;    cystic fibrosis;    CFTR;    cystic fibrosis transmembrane conductance regulator;    PCD;    programmed cell death;    TUNEL;    terminal deoxynucleotidyl transferase-mediated d-UTP-digoxygenin nick-end labelling;   

DOI  :  10.1016/S0014-5793(97)00347-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30–82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1–7) in controls P<0.001) and median 37.5% (range: 23–79) crypt enterocytes show Ki67 antigen (P<0.001). In CF airways 57% (range: 54–70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

【 授权许可】

Unknown