Molecular heterogeneity of the cDNA encoding a 74‐kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A

FEBS Letters

Tanabe, Osamu¹  Takeda, Masao¹  Gomez, Gloria A¹  Nishito, Yasumasa¹  Usui, Hirofumi¹ 

[1] Department of Biochemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan

关键词: Protein phosphatase 2A;    74-kDa regulatory subunit (B″ or δ);    Human erythrocyte;    Human cerebral cortex;    Splicing variant;    B′ subunit;    ABRPP2A;    protein phosphatase 2A;    PCR;    polymerase chain reaction;    KLH;    keyhole limpet hemocyanin;    MBS;    m-maleimidobenzoyl-N-hydoxysuccinimide ester;    PAGE;    polyacrylamide gel electrophoresis;    bp;    base pair;    kb;    kilobase;   

DOI  :  10.1016/S0014-5793(97)00392-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two cDNAs for possible splicing variants of a 74-kDa regulatory subunit (B″ or δ) of human protein phosphatase 2A, were isolated. These variants were identified from human cerebral cortex by library screening and PCR, and designated δ1 and δ3 isoforms, while the previously reported isoform [Tanabe et al. (1996) FEBS Lett. 379, 107–111] was designated δ2. Compared with the δ2 isoform, the δ1 isoform contained a 32-residue insertion beginning at residue 84, and consisted of 602 amino acids in all. The δ3 isoform lacked a 74-residue sequence corresponding to residues 1083 of the δ2 isoform, and consisted of 496 amino acids. Using isoform-specific antipeptide antisera, the 74-kDa subunit (B″ or δ) originally purified from human erythrocytes was identified as the δ1 isoform.

【 授权许可】

Unknown