DNA binding sites recognised in vitro by a knotted class 1 homeodomain protein encoded by the hooded gene, k, in barley (Hordeum vulgare)

FEBS Letters

Gausing, Kirsten¹  Krusell, Lene¹  Rasmussen, Inge¹ 

[1] Department of Molecular and Structural Biology, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: Homeobox gene;    Transcription factor;    cDNA sequence;    Gel shift;    Barley;    Maize;    ITOG;    isopropyl β-d-thiogalactoside;   

DOI  :  10.1016/S0014-5793(97)00382-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The homeodomain of the knotted classes of transcription factors from plants differs from the well characterized Antp/En type homeodomains from Drosophila at key amino acid residues contributing to the DNA binding. A cDNA, Hvh21, derived from the hooded gene and encoding a full length homolog of knotted1 from maize was isolated from barley seedlings and expressed as a maltose binding protein fusion in E. coli. The purified HvH21-fusion protein selected DNA fragments with 1–3 copies of the sequence TGAC. Gel shift experiments showed that the TGAC element was required for binding and the results further indicate that the HvH21-fusion protein binds DNA as a monomer.

【 授权许可】

Unknown