Deletion in the Z‐line region of the titin gene in a baby hamster kidney cell line, BHK‐21‐Bi

FEBS Letters

Jäckel, Monika¹  Witt, Christian¹  Curdt, Ingo¹  Antonova, Olga¹  Jockusch, Harald¹  Labeit, Siegfried² 

[1] Developmental Biology Unit, University of Bielefeld, D-33501 Bielefeld, Germany;Biological Structures and Biocomputing Programme (EMBL), D-69012 Heidelberg, Germany

关键词: Cytoskeleton;    Sarcomere;    Permanent cell line;    Genetic deterioration;   

DOI  :  10.1016/S0014-5793(97)00381-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titin non-functional. These sequences were present in the original BHK-21 line and in hamster DNAs. Our finding shows that, due to the absence of selective pressure on a gene's function, genomic deterioration can occur in a permanent cell line and can lead to a loss of overlapping DNA stretches in both autosomes.

【 授权许可】

Unknown