Spin trapping agent phenyl N‐tert‐butylnitrone protects against the onset of drug‐induced insulin‐dependent diabetes mellitus

FEBS Letters

Tabatabaie, Tahereh¹  Floyd, Robert A¹  Wallis, Gemma¹  Jacob, Jane M²  Kotake, Yashige¹ 

[1] Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, 825 Northeast, 13th Street, Oklahoma City, OK 73104, USA;Department of Anatomical Sciences, University of Oklahoma, Health Sciences Center, Oklahoma City, OK 73190, USA

关键词: Diabetes;    Streptozotocin;    Phenyl N-tert-butylnitrone;    Nitric oxide;    Electron paramagnetic resonance;    Spin trap;    STZ;    streptozotocin;    NO;    nitric oxide;    PBN;    phenyl N-tert-butylnitrone;    IL-1;    interleukin-1;    iNOS;    inducible nitric oxide synthase;    EPR;    electron paramagnetic resonance;    Fe-DTCS;    iron complex of N-(dithiocarboxy) sarcosine;   

DOI  :  10.1016/S0014-5793(97)00327-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin-dependent diabetes mellitus is an autoimmune disease believed to be caused by an inflammatory process in the pancreas leading to selective destruction of the β-cells. Cytokines and nitric oxide (NO) have been shown to be involved in this destruction. Phenyl N-tert-butylnitrone (PBN) has demonstrated protective effects against several pathological conditions including ischemia-reperfusion injury and endotoxin-induced shock. We report here that PBN co-administration can prevent the onset of the STZ-induced diabetes in mice. PBN co-treatment inhibited the streptozotocin (STZ)-induced hyperglycemia, the elevation in the level of glycated hemoglobin and weight loss in the treated mice. Histological observations indicated destruction of β-cells in the STZ-treated animals and its prevention by PBN co-treatment. EPR spin trapping experiments in the pancreas indicated the in vivo formation of NO in STZ-treated animals and its attenuation by PBN treatment.

【 授权许可】

Unknown