The S.//.A.IG amino acid motif is present in a replication dependent late H3 histone variant of P. lividus sea urchin

FEBS Letters

Forte, Amalia¹  Aniello, Francesco²  Affaitati, Adele²  Fucci, Laura¹  Branno, Margherita²  Geraci, Giuseppe¹  Mancini, Pietro¹ 

[1] Department of Genetics, General and Molecular Biology, University of Naples `Federico II', Via Mezzocannone 8, 80134 Naples, Italy;Stazione Zoologica `Anton Dohrn', Villa Comunale, Naples, Italy

关键词: H3 histone;    Replication dependent variant;    Late histone;    Sea urchin embryo;    P. lividus;   

DOI  :  10.1016/S0014-5793(97)00305-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel gene encoding a new H3 histone variant (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3′ terminal stem-loop nucleotide sequence that is typical of the replication dependent variants. The gene is intronless, the corresponding short transcript is non-polyadenylated and its expression is replication dependent with a timing of a late variant. The new H3 variant is expressed as a minor component with respect to a major replication dependent late H3 histone here identified by partial cDNA sequence. These results show that classification of histones in replication dependent and independent variants only on the basis of their amino acid sequences should be reconsidered.

【 授权许可】

Unknown