Identification of a vitamin K‐dependent carboxylase in the venom duct of a Conus snail

FEBS Letters

Bandyopadhyay, Pradip K¹  Olivera, Baldomero M¹  Stanley, Thomas B²  Stafford, Darrel W² 

[1] Department of Biology, University of Utah, Salt Lake City, UT 84112, USA;Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA

关键词: Vitamin K-dependent glutamyl carboxylase;    Gamma-carboxyglutamate;    Post-translational modification;    Conus radiatus;   

DOI  :  10.1016/S0014-5793(97)00299-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peptides from the venom ducts of cone snails (genus Conus) contain γ-carboxyglutamate residues. The γ-glutamyl carboxylase responsible for this post-translational modification is localized in the microsomal fraction, strictly dependent on vitamin K, activated by ammonium sulfate, and is associated with endogenous substrate. The K _m of the enzyme for vitamin K is comparable to that for the bovine carboxylase. However, a propeptide containing substrate related to the blood coagulation protein factor IX, a highly efficient substrate for the bovine enzyme, was poorly carboxylated by the Conus enzyme, suggesting differences in γ-carboxylase recognition signal sequences and/or structural requirements at the carboxylation site.

【 授权许可】

Unknown