Carbohydrate induced modulation of cell membrane. VI. Binding of exogenous lectin induces susceptibility of erythrocytes to free radical damage: a spin label study

FEBS Letters

Hajela, Krishnan¹  Sumati,  ¹  Pande, Abhay H¹ 

[1]School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Khandwa Road Campus, Indore 452001 (M.P.), India

关键词: Lectin;    Red blood cell membrane;    Pyrogallol;    Electron paramagnetic resonance;    16-DS;    16-doxyl stearic acid;    PBN;    N-t-butyl-phenyl nitrone;    PBS;    0.01 M phosphate buffer pH 7.4 with 0.15 M NaCl;    EPR;    electron paramagnetic resonance;   

DOI  :  10.1016/S0014-5793(97)00272-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The oxidation of erythrocyte membrane has been widely used as a model to study the damage of biomembranes by free radicals. Whether binding of lectin to erythrocytes has any effect on peroxidant injury has never been studied. This study reports for the first time that crosslinking of erythrocyte surface glycoprotein by an exogenous lectin significantly enhances the susceptibility to membrane damage by free radicals, as evidenced by the increase in membrane fluidity measured by EPR using spin label and the increase in the amount of oxyhemoglobin liberated due to cell lysis.

【 授权许可】

Unknown