c‐Ras is required for the activation of the matrix metalloproteinases by concanavalin A in 3Y1 cells

FEBS Letters

Thant, Aye Aye²  Serbulea, Mihaela¹  Kikkawa, Fumitaka²  Liu, Enbo²  Hamaguchi, Michinari³  Tomoda, Yutaka² 

[1] Department of Internal Medicine III, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan;Department of Obstetrics and Gynaecology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan;Department of Molecular Pathogenesis, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan

关键词: Matrix metalloproteinase;    MMP-2;    MMP-9;    ras;    Concanavalin A;    Signal transduction;   

DOI  :  10.1016/S0014-5793(97)00230-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Concanavalin A (Con A) is known to trigger augmented secretion and proteolytic activation of the matrix metalloproteinases (MMPs) in fibroblasts. To study the signaling pathway critical for the activation of MMPs in fibroblasts, we examined the effects of dominant negative ras (S17N ras) expression under the control of conditionally inducible promoter in Con A-activated 3Y1 cells. We found that augmented secretion and proteolytic activation of MMP-2 and MMP-9 together with expression of MT1-MMP in Con A-activated 3Y1 were dramatically suppressed by S17N ras expression. These results strongly suggest that c-Ras plays a critical role in the augmented expression and proteolytic activation of MMPs in fibroblasts.

【 授权许可】

Unknown