Role of Csk in neural differentiation of the embryonic carcinoma cell line P19

FEBS Letters

Okada, Masato¹  Takayama, Yoshiharu¹  Nada, Shigeyuki¹  Nagai, Katsuya¹ 

[1] Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565, Japan

关键词: Csk;    Src family kinases;    Neuronal differentiation;    P19 cells;    L1;   

DOI  :  10.1016/S0014-5793(97)00224-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To examine the neural function of Csk (C-terminal Src kinase), a membrane-targeted form of Csk (Src/Csk) and its kinase-defective variant (DK-Src/Csk) were expressed in the embryonic carcinoma cell line P19. Expression of Src/Csk, but not DK-Src/Csk, caused reduction of the specific activities of Src and Fyn in the differentiated P19 cells. During neural differentiation, the specific activity of Src was elevated in the control P19 cells, whereas the activation was completely eliminated in the Src/Csk transfectant. In normally differentiated P19 cells, cross-linking of a cell adhesion molecule, L1, induced a short-term activation of Src and Fyn. In the Src/Csk transfectant, L1 stimulation induced delayed activation of Src and Fyn peaking at much lower levels than in the control cells. Src/Csk transfectants developed normally in the initial stages of neural differentiation, but exhibited an apparent defect in cell-to-cell interaction, i.e. neurite fasciculation and aggregation of cell bodies, in the latter stages. These findings imply that Csk is involved in the regulation of Src family kinases that play roles in cell-to-cell interaction mediated by cell adhesion molecules.

【 授权许可】

Unknown