Engineering of diffraction‐quality crystals of the NF‐κB P52 homodimer:DNA complex

FEBS Letters

Cramer, Patrick¹  Müller, Christoph W¹ 

[1] European Molecular Biology Laboratory (EMBL), Grenoble Outstation, c/o ILL, B.P. 156, 38042 Grenoble Cedex 9, France

关键词: Rel protein;    NF-κB P52;    Protein:DNA interaction;    Co-crystallization;    Transcription;    Protein engineering;    BSA;    bovine serum albumin;    DLS;    dynamic light scattering;    DTT;    dithiothreitol;    EMSA;    electrophoretic mobility shift assay;    ESRF;    European Synchrotron Radiation Facility;    FPLC;    fast protein liquid chromatography;    HIV;    human immunodeficiency virus;    IPTG;    isopropyl-β-d-thiogalactopyranoside;    MES;    2-[N-morpholino]ethanesulfonic acid;    NLS;    nuclear localization signal;    PEG;    polyethylene glycol;    PMSF;    phenylmethyl sulfonyl fluoride;    RHR;    Rel homology region;   

DOI  :  10.1016/S0014-5793(97)00217-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown