Engineering thermolysin‐like proteases whose stability is largely independent of calcium

FEBS Letters

Venema, Gerard¹  Veltman, Oene R¹  Hardy, Florence¹  van den Burg, Bertus¹  Eijsink, Vincent G.H²  Vriend, Gert³ 

[1] Department of Genetics, Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands;Laboratory of Microbial Gene Technology, NLVF, PO Box 5051, 1432 Ås, Norway;EMBL, BIOcomputing, Meyerhofstrasse 1, 69117 Heidelberg, Germany

关键词: Calcium binding;    Thermal stability;    Thermolysin;    Autolysis;    Unfolding pathway;   

DOI  :  10.1016/S0014-5793(97)00193-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown