Deletion mutagenesis as a test of evolutionary relatedness of indoleglycerol phosphate synthase with other TIM barrel enzymes

FEBS Letters

Dahm, Anke¹  Kirschner, Kasper¹  Stehlin, Catherine² 

[1] Department of Biophysical Chemistry, Biozentrum of the University, CH 4056 Basel, Switzerland;Department of Chemistry, University of Minnesota, Minneapolis MN 55455, USA

关键词: Protein evolution;    Protein engineering;    Thermostability;    TIM barrel protein;    Indoleglycerol phosphate synthase;    DTE;    dithioerythritol;    HEPPS;    N-(2-hydroxyethyl)piperazine N′-(3-propanesulfonic acid);    GdmCl;    guanidinium chloride;    PMSF;    phenylmethyl sulfonyl fluoride;    CdRP;    1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate;    IGP;    indoleglycerol phosphate;    IGPS;    indoleglycerol phosphate synthase;    PRAI;    phosphoribosyl anthranilate isomerase;    eIGPS-PRAI;    bifunctional enzyme from E. coli;   

DOI  :  10.1016/S0014-5793(97)00066-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown