19F‐NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles

FEBS Letters

Lin, Chan-Lan S¹  Rosenberger, Michael²  Li, Ellen¹  Rong, Ding¹  d'Avignon, D.Andre³  Lovey, Allen J² 

[1] Department of Medicine, Washington University, School of Medicine, 660 South Euclid Avenue, Box 8051, St. Louis, MO 63110, USA;Hoffmann-La Roche Inc., Nutley, NJ 07110, USA;Department of Chemistry, Washington University, St. Louis, MO 63110, USA

关键词: Cellular retinol binding protein;    Cellular retinol binding protein II;    Retinol;    Liposome;    19F-NMR;    CRBP;    cellular retinol binding protein;    CRBP II;    cellular retinol binding protein II;    NMR;    nuclear magnetic resonance;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PS;    phosphatidylserine;    D2O;    deuterium oxide;    CDCl3;    chloroform-d;    6-FTrp;    6-fluorotryptophan;    TMS;    trimethylsilane;    TFA;    trifluoroacetic acid;    FABP;    fatty acid binding protein;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(96)01509-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. ¹⁹F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.

【 授权许可】

Unknown