Nuclear translocation of PKCζ during ischemia and its inhibition by wortmannin, an inhibitor of phosphatidylinositol 3‐kinase

FEBS Letters

Mizukami, Yoichi¹  Hirata, Takao¹  Yoshida, Ken-ichi¹ 

[1] Department of Legal Medicine, Yamaguchi University School of Medicine, 1144 Kogushi, Ube 755, Japan

关键词: Protein kinase Cζ;    Ischemia;    Wortmannin;    Rat heart;    PKC;    protein kinase C;    cPKC;    conventional PKC;    nPKC;    novel PKC;    aPKC;    atypical PKC;    DG;    diacylglycerol;    PI3-kinase;    phosphatidylinositol 3-kinase;    PIP3;    phosphatidylinositol-3;    4;    5-triphosphate;    PIP2;    phosphatidylinositol-4;    5-bisphosphate;    PS;    phosphatidylserine;    WT;    wortmannin;    PBS;    phosphate buffered saline;    ECL;    enhanced chemiluminescence;    MEK-1;    mitogen-activated protein kinase/extracellular regulated protein kinase kinase-1;   

DOI  :  10.1016/S0014-5793(96)01481-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase Cζ (PKCζ), a member of the atypical PKC subgroup, is insensitive to Ca²⁺, diacylglycerol, and phorbol esters, but is activated by phospholipids such as phosphatidylinositol-3,4,5-triphosphate, a product of phosphatidylinositol 3-kinase (PI3-kinase). Here we show that PKCζ translocates from the cytosol to the 1000×g pellet (nuclear-myofibrillar) fraction during ischemia for 40 min in Langendorff-perfused rat hearts. In addition, immunohistochemical observation shows that ischemia induces the translocation of PKCζ to the nucleus. The nuclear translocation during ischemia is inhibited in a dose-dependent manner by wortmannin (10⁻⁹–10⁻⁷ M), an inhibitor of PI3-kinase.

【 授权许可】

Unknown