Rat prolactin synthesis by lactating mammary epithelial cells

FEBS Letters

Le Provost, Fabienne⁴  Delpal, Serge¹  Ollivier-Bousquet, Michèle²  Lkhider, Mustapha³ 

[1] Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Jouy-en-Josas, France;Laboratoire de Biologie Cellulaire et Moléculaire, INRA, Jouy-en-Josas, France;Université Chouaib Doukkali, Faculté des Sciences, El Jadida, Morocco;Laboratoire de Génétique Biochimique et Cytogénétique, INRA, Jouy-en-Josas, France

关键词: Prolactin;    Synthesis;    Mammary epithelial cell;    Lactation;    Bromocriptine;   

DOI  :  10.1016/S0014-5793(96)01450-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [³⁵S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cycloheximide. Immunoreactive 14-, 23-, 25-, 32- and 36-kDa PRL forms were radioactive. By two-dimensional electrophoresis analysis, immunoreactive and radioactive spots, of about 25 kDa and high molecular weight, were also detected. After incubation of mammary epithelial cells with cycloheximide, immunogold electron microscopy showed a drastic decrease of labelling in organelles involved in synthesis and secretion, compared to those incubated in control medium. These results make it possible to conclude that lactating mammary tissue is able to synthesize PRL.

【 授权许可】

Unknown