Binding of the N‐terminal 63 kDa portion of connectin/titin to α‐actinin as revealed by the yeast two‐hybrid system

FEBS Letters

Ohtsuka, Hiroshi¹  Maruyama, Koscak¹  Kimura, Sumiko¹  Yajima, Hirohiko¹ 

[1] Department of Biology, Faculty of Science, Chiba University, Chiba 263, Japan

关键词: Connectin;    Titin;    α-Actinin;    Muscle structure;    Z-line;    Two-hybrid system;    Enzyme-linked immunosorbent assay;   

DOI  :  10.1016/S0014-5793(96)01432-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones coding the C-terminal half region of α-actinin (amino acids, 343–897 and 446–897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of α-actinin and the N-terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N-terminal 63 kDa portion of connectin binds to α-actinin in the Z-line of myofibrillar sarcomeres.

【 授权许可】

Unknown