Paramagnetic NMR spectroscopy of native and cobalt substituted manganese superoxide dismutase from Escherichia coli

FEBS Letters

Renault, Jean Philippe¹  Morgenstern-Badarau, Irène¹  Piccioli, Mario²  Verchère-Béaur, Catherine¹ 

[1]Laboratoire de Chimie Bioorganique et Bioinorganique, Institut de Chimie Moléculaire d'Orsay, Université Paris-Sud, 91405 Orsay cedex, France

[2]Department of Chemistry, University of Florence, Via Gino Capponi 7, Florence, Italy

关键词: Superoxide dismutase;    Manganese;    Cobalt;    Paramagnetic NMR;    Escherichia coli;    SOD;    superoxide dismutase;    MnSOD;    manganese containing superoxide dismutase;    FeSOD;    iron containing superoxide dismutase;   

DOI  :  10.1016/S0014-5793(96)31420-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Manganese containing superoxide dismutase from E. coli has been investigated through paramagnetic NMR spectroscopy. The spectrum of the native form was rationalized using a τ_s=3×10⁻¹¹ s for the Mn(III) ion, consistent with previous estimates from NMRD measurements. Mn(III) has been replaced by a Co(II) ion and a tentative assignment of the NMR spectrum of the Co(II)-substituted derivative has been proposed, based on T₁, chemical shifts and 1D-NOE data. The metal coordination geometry is provided by three histidines and a carboxylate group. The presence of a solvent molecule as a loosely bound fifth ligand is also proposed. The NMR data of the Co(II)-substituted derivative of E. coli MnSOD differs from those of Co(II)SOD from other bacterial sources. This suggests that Co(II) substitution is an efficient method to address the problem of metal ion selectivity in superoxide dismutase.

【 授权许可】

Unknown