A model for Fis N‐terminus and Fis‐invertase recognition

FEBS Letters

Hwang, Ming-Jing¹  Tzou, Wen-Shyong² 

[1] Institute of Biomedical Sciences, Academia Sinica, 128 Yen-chiou Yuan Rd., Sec. 2, Taipei 11529, Taiwan;Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan

关键词: Fis;    Invertase;    Structure prediction;    Homology modeling;   

DOI  :  10.1016/S0014-5793(96)01348-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this modeling, we show that the elusive Fis N-terminus involved in invertase-mediated site-specific DNA inversion is, by all indications, a hinged flapping loop. The prediction is based on a combined sequence and secondary structure alignment against known structures of protein segments, as well as a tetrapeptide fragment observed crystallographically. Its validity is strongly supported by the ability to interpret consistently the available mutagenesis data pertaining to this region including, especially, a series of deletion mutants which until this work had been a puzzle in the search for structural explanations. A model for Fis-invertase recognition is also proposed.

【 授权许可】

Unknown