FEBS Letters | |
A model for Fis N‐terminus and Fis‐invertase recognition | |
Hwang, Ming-Jing1  Tzou, Wen-Shyong2  | |
[1] Institute of Biomedical Sciences, Academia Sinica, 128 Yen-chiou Yuan Rd., Sec. 2, Taipei 11529, Taiwan;Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan | |
关键词: Fis; Invertase; Structure prediction; Homology modeling; | |
DOI : 10.1016/S0014-5793(96)01348-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In this modeling, we show that the elusive Fis N-terminus involved in invertase-mediated site-specific DNA inversion is, by all indications, a hinged flapping loop. The prediction is based on a combined sequence and secondary structure alignment against known structures of protein segments, as well as a tetrapeptide fragment observed crystallographically. Its validity is strongly supported by the ability to interpret consistently the available mutagenesis data pertaining to this region including, especially, a series of deletion mutants which until this work had been a puzzle in the search for structural explanations. A model for Fis-invertase recognition is also proposed.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303804ZK.pdf | 776KB | download |