Isolation and characterization of the bioactive circulating human parathyroid hormone, hPTH‐1–37

FEBS Letters

Heine, Gabi¹  Mägerlein, Markus²  Forssmann, Wolf-Georg¹  Hock, Dieter¹  Ochlich, Peter P² 

[1] Niedersächsisches Institut für Peptid-Forschung (IPF), Feodor-Lynen-Str. 31, D-30625 Hannover, Germany;Boehringer Mannheim GmbH, Sandhofer Str. 116, D-68305 Mannheim, Germany

关键词: Parathyroid hormone;    Isolation;    Hemofiltrate;    Dialysis;    Human;   

DOI  :  10.1016/S0014-5793(96)01390-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The occurrence of hPTH-1–37 as the native bioactive circulating form of PTH-1–84 has now been obtained using a specific purification procedure for circulating parathyroid hormone, which involves a newly developed immunoenzymetric assay for N-terminally intact hPTH. In combination with two different methods of mass spectrometry, the molecular weight of the isolated immunoreactive peptide was shown to be 4401 Da, which corresponds to hPTH-1–37. Synthetic hPTH-1–37 material was tested in the chick bioassay and produced a clear-cut increase in serum calcium concentration. We conclude that hPTH-1–37 is the native bioactive fragment of hPTH-1–84 in circulation.

【 授权许可】

Unknown