Aluminum interaction with plasma membrane lipids and enzyme metal binding sites and its potential role in Al cytotoxicity

FEBS Letters

Jones, David L¹  Kochian, Leon V² 

[1] School of Agricultural and Forest Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK;US Plant Soil and Nutrition Laboratory, USDA-ARS, Cornell University, Ithaca, NY 14850, USA

关键词: Aluminium;    Cytotoxicity;    Enzyme activity;    Lipid binding;    Metal binding;    Zinc;   

DOI  :  10.1016/S0014-5793(96)01319-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The trivalent cation aluminum can cause chronic cytotoxicity in plants, animals and microorganisms. It has been suggested that Al interaction with cell membranes and enzyme metal binding sites may be involved in Al cytotoxicity. In this study, the binding of Al to microsomes and liposomes was found to be lipid dependent with the signal transduction element phosphatidylinositol-4,5-bisphosphate having the highest affinity for Al with an Al:lipid stoichiometry of 1:1. Al binding was only reduced in the presence of high concentrations of Ca²⁺ (>1 mM). Both citrate and, to a lesser extent, malate were capable of preventing Al lipid binding, which is consistent with the involvement of these organic acids in a recently described Al detoxification mechanism in plants. The effects of AlCl₃, Al-citrate and ZnSO₄ on metal-dependent enzyme activities (enolase, pyruvate kinase, H⁺-ATPase, myosin, Calpain, proteinase K, phospholipase A₂ and arginase) was assayed in vitro. While Zn²⁺ was capable of inhibiting all the enzymes except the H⁺-ATPase, AlCl₃ and Al-citrate had minimal effects except for with phospholipase A₂ where an interaction with AlCl₃ occurred. However, this could be negated by the addition of citrate. The results indicate that, contrary to current hypotheses, the toxic mode of Al is not through an interaction with enzymatic catalytic metal binding sites but may be through the interaction with specific membrane lipids.

【 授权许可】

Unknown