Expression of a transactivation‐deficient form of thyroid transcription factor I decreases the activity of co‐transfected thyroglobulin and thyroperoxidase promoters

FEBS Letters

Christophe, Daniel¹  Christophe-Hobertus, Christiane¹  Pichon, Bruno¹  van Renterghem, Pierre¹ 

[1] I.R.I.B.K.N., Université Libre de Bruxelles, Fuculté de Médecine, Route de Lennick 808, Bât. C, 1070 Brussels, Belgium

关键词: TTF-1;    Homeodomain;    Thyroglobulin;    Thyroperoxidase;    Transcription;    Thyroid;   

DOI  :  10.1016/S0014-5793(96)01308-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thyroid transcription factor I (TTF-1) plays a critical role in thyroid organogenesis and in the control of expression of several thyroid-specific genes, like those coding for thyroglobulin and thyroperoxidase. We have expressed the isolated DNA-binding homeodomain of TTF-1 in cultured thyroid cells by transient transfection. A specific reduction in the activity of cotransfected thyroglobulin and thyroperoxidase promoters was observed in the presence of the isolated TTF-1 homeodomain, as compared to their activity measured in the presence of a mutated homeodomain unable to bind DNA. The activity of the SV40 early promoter, used as a control, was only marginally affected in these experiments. The transactivation-deficient form of TTF-1 described here may thus be used for investigating other cellular processes that are dependent on TTF-1 transcriptional activity.

【 授权许可】

Unknown