Cloning and functional analysis of the hematopoietic cell‐specific phospholipase Cγ 2 promoter

FEBS Letters

Hug, Hubert⁴  Kohlhuber, Franz²  Ueffing, Marius²  Eick, Dirk²  Marmé, Dieter¹  Kang, Jong Sun³ 

[1] Institut für Molekulare Medizin and Naturstofforschung, Klinik für Tumorbiologie, Breisacher Straβe 117, 79106 Freiburg, Germany;Institut für Klinische Molekularbiologie and Tumorgenetik, GSF, Marchioninistr. 25, 81377 München, Germany;Mount Sinai Medical School, Department of Biochemistry, 1 Gustave Levy Place, New York, NY 10029, USA;ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany

关键词: Phospholipase Cγ 2 (PLCγ 2);    Promoter;    Gene regulation;    SP1;    B-cell;   

DOI  :  10.1016/S0014-5793(96)01276-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipase Cγ ² (PLCγ ²) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCγ ¹, PLCγ ² is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is activated by tyrosine phosphorylation after the triggering of B-cell surface immunoglobulin. We have cloned and sequenced the human PLCγ ² promoter. Primer extension analysis reveals the existence of a major transcriptional start site. The TATA-less promoter contains G+C-rich stretches with a cluster of contiguous SP1 consensus sites, an NF1, and an AP2 site between by −220 to −70. A construct containing the region from −189 to +78 confers full promoter activity, as shown by fusion to a luciferase reporter gene construct. The distal part of the promoter between by −662 to −293 containing an SRE, EBF and CACCC box contributed negatively to promoter activity in the B-cell line Raji but not in three adherent cell lines. In Raji cells, PLCγ ² mRNA is expressed at low levels with a half life greater than 4 h. After treatment with serum, TPA, retinoic acid, or with 5-azacytidine increased levels of PLCγ ² mRNA were induced in B-cells.

【 授权许可】

Unknown