Molecular cloning and nucleotide sequence of the gene encoding phosphate‐inducible pectin lyase of Bacillus subtilis

FEBS Letters

Sakamoto, Tatsuji¹  Kawasaki, Haruhiko¹  Sakai, Takuo² 

[1] Department of Applied Biological Chemistry, College of Agriculture, Osaka 593, Japan;Department of Food Science, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631, Japan

关键词: Pectin lyase;    Protopectinase;    Nucleotide sequence;    Phosphate-dependent expression;    Bacillus subtilis;    IPTG;    Isopropyl-1-thio-β-d-galactopyranoside;   

DOI  :  10.1016/S0014-5793(96)01257-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding the pectin lyase (PNL; EC 4.2.2.10) of Bacillus subtilis has been cloned, sequenced, and characterized. A coding sequence for the PNL composed of 345 amino acids including a 24-amino-acid signal peptide was assigned. No sequence resembling a LexA binding site was found upstream of the structural gene. Furthermore, PNL activity of the gene product expressed in Escherichia coli DH5α was detected intracellularly, which might suggest that expression of the gene was not controlled by RecA. Regulation of the gene expression seemed to be quite different from that of other bacterial PNL genes previously reported.

【 授权许可】

Unknown