Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters

FEBS Letters

Athena Guo, W.-X.¹  Merlie, John P.¹  Nichol, Mia¹ 

[1] Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA

关键词: Utrophin;    Dystrophin;    Acetylcholine receptor;    Neuromuscular junction;    cDNA cloning;    Gene expression;   

DOI  :  10.1016/S0014-5793(96)01216-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced mouse utrophin cDNA, and successfully expressed full length utrophin (400 kDa) in both muscle and non-muscle cells. The expression of recombinant utrophin is compared with that of its homologue, dystrophin (427 kDa). We demonstrate that recombinant utrophin is targeted into agrin-induced acetylcholine receptor (AChR) clusters, while recombinant dystrophin is evenly distributed along cell membranes in cultured Sol 8 muscle cells. This observation suggests that utrophin and dystrophin may interact with different cytoskeletal proteins. The C-terminal domains are found to be responsible for the association of utrophin with AChR clusters.

【 授权许可】

Unknown