FEBS Letters | |
The light intensity dependence of protochlorophyllide photoconversion and its significance to the catalytic mechanism of protochlorophyllide reductase | |
Blankenship, Robert E.2  McHugh, Thomas2  Griffiths, W.Trevor1  | |
[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK;Department of Biochemistry and Chemistry, Arizona State University, Tempe, AZ 85287-1604, USA | |
关键词: Protochlorophyllide reductase; Light intensity dependence; Photoenzyme mechanism; | |
DOI : 10.1016/S0014-5793(96)01249-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
The light-dependent step in chlorophyll synthesis by higher plants involves hydrogen transfer from NADPH+ to the porphyrin protochlorophyllide catalysed by the photoenzyme protochlorophyllide reductase. The light intensity dependence of the process has been studied in vitro using wheat etioplast membranes. The results suggest that a single photochemical event is involved in the photoconversion. In support of this conclusion we also demonstrate that illumination of these membranes with a train of ultrashort (150 fs) flashes resulted in chlorophyll accumulation. The significance of the findings in terms of possible mechanisms for the reductase are discussed.
【 授权许可】
Unknown
【 预 览 】
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