The light intensity dependence of protochlorophyllide photoconversion and its significance to the catalytic mechanism of protochlorophyllide reductase

FEBS Letters

Blankenship, Robert E.²  McHugh, Thomas²  Griffiths, W.Trevor¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK;Department of Biochemistry and Chemistry, Arizona State University, Tempe, AZ 85287-1604, USA

关键词: Protochlorophyllide reductase;    Light intensity dependence;    Photoenzyme mechanism;   

DOI  :  10.1016/S0014-5793(96)01249-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The light-dependent step in chlorophyll synthesis by higher plants involves hydrogen transfer from NADPH⁺ to the porphyrin protochlorophyllide catalysed by the photoenzyme protochlorophyllide reductase. The light intensity dependence of the process has been studied in vitro using wheat etioplast membranes. The results suggest that a single photochemical event is involved in the photoconversion. In support of this conclusion we also demonstrate that illumination of these membranes with a train of ultrashort (150 fs) flashes resulted in chlorophyll accumulation. The significance of the findings in terms of possible mechanisms for the reductase are discussed.

【 授权许可】

Unknown