Bandeiraea (Griffonia) simplicifolia lectin‐I, isolectin A4, reacting with Tn (GalNAαl → Ser/Thr or galabiose (Galα1 → 4Gal) containing ligands

FEBS Letters

Albert M., Wu³  Kabat, Elvin A.²  June H., Wu¹ 

[1] Department of Microbiology and Immunology, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan;Department of Microbiology, Human Genetics and Development, and Neurology, and Cancer Center/Institute for Cancer Research, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA;Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan

关键词: Bandeiraea (Griffonia) simplicifolia lectin-I A4;    Tn-active glycoprotein;    Sheep hydatid cyst glycoprotein;    Binding property;    Gal;    d-galactopyranose;    Glc;    d-glucopyranose;    lFuc or Fuc;    l-fucopyranose;    GalNAc;    2-acetamido-2-deoxy-d-galactopyranose;    GlcNAc;    2-acetamido-2-deoxy-d-glucopyranose;    GS I-A4 (BS I-A4);    Griffonia (Bandeiraea) simplicifolia lectin-I A4;    GP;    glycoprotein;    THGP;    Tamm-Horsfall glycoprotein;    MSM;    one of human blood group A active glycoproteins purified from human ovarian cyst fluid [26];    OSM;    ovine submandibular glycoprotein-major;    ASG-A;    armadillo submandibular glycoprotein fraction A [17] which is one of the sialic acid containing glycoproteins in armadillo submandibular glands;    native ASG-Tn [18];    a naturally occurring Tn glycoprotein isolated from the extract of armadillo submandibular glands in 0.01 M PBS pH 6.8 after removal of ASG-A;    PSM;    porcine salivary GPmajor;    BSM;    bovine submandibular GP-major;    QPA;    quantitative precipitin assay;    QPIA;    quantitative precipitin-inhibition assay. Lectin determinants that are used to classify applied lectins [14;    30;    31] are expressed in bold: A(GalNAcα1 → 3Gal);    b(Galα1 → 3Gal);    Tn(GalNAcα1 → Ser/Thr);    E(galabiose Galα1 → 4Gal) [30;    31];    the human blood group Pk(Galα1 → 4Galβ1 → 4Glc-Cer) active dissacharide or part of P1 determinant structure (Galα1 → 4Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Gcl-Cer) at the terminal non-reducing end;   

DOI  :  10.1016/S0014-5793(96)01227-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A₄(GS I-A₄) reacting with the Tn(GalNAcα1 → Ser/Thr) sequence or human blood group P ^k active dissacharide (E, Galα1 → 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P₁ or Tn active glycoproteins were tested by QPA, GS IA₄ reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P₁ active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A₄ interaction was inhibited by both Tn containing glycopeptides and Galα1 → 4Gal indicating that GS I-A₄ not only reacts with human blood group A(GalNAcα1 → 3Gal) and B(Galα1 → 3Gal) active dissacharides, but also recognizes the Tn sequence and the E(Galα1 → 4Gal) ligand. From these results, the carbohydrate specificity of GS I-A₄ can be defined as A, Tn ≥ B and E.

【 授权许可】

Unknown