ATF site of human RB gene promoter is a responsive element of myogenic differentiation

FEBS Letters

Fujita, Tsuyoshi⁴  Endo, Takeshi²  Sakai, Toshiyuki⁴  Nomura, Hitoshi³  Mizuno, Takakazu³  Nikaido, Toshio¹  Sowa, Yoshihiro⁴  Okuyama, Yusuke⁴ 

[1] Department of Gynecology and Obstetrics, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390, Japan;Department of Biology, Faculty of Science, Chiba University, Yayoicho, Inage-ku, Chiba 263, Japan;Chugai Research Institute for Molecular Medicine, Inc., 153-2 Nagai, Niihari-mura, Niihari-gun, Ibaraki 300-41, Japan;Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

关键词: RB gene promoter;    Myogenic differentiation;    ATF;   

DOI  :  10.1016/S0014-5793(96)01178-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RB mRNA increases during terminal differentiation of C2 myoblasts. We demonstrate that RB promoter activity increases about 4-fold during differentiation. The increase of RB promoter activity was reduced when a point mutation was designed in the ATF site. In a gel shift assay of the ATF site, two specific bands were observed. One of them, with the lower mobility, disappeared during differentiation. This band reacted with an antibody against ATF-1. We cotransfected an RB promoter-luciferase plasmid with the TREB36/ATF-1 plasmid. ATF-1 suppressed the activity of the wild-type RB promoter but not of that with a point mutation at the ATF site. These results suggest that the ATF site of the RB promoter is a responsive element during myogenic differentiation of C2 cells. We hypothesize that RB promoter activity is stimulated partially due to the dissociation of ATF-1, which suppresses the promoter activity through the ATF site in C2 myoblasts.

【 授权许可】

Unknown