Testing the eukaryotic promoters for efficient expression of exogenous genes in chondrocytes and synoviocytes

FEBS Letters

Zafarullah, Muhammad²  Dehnade, Faramaze¹  Su, Suming²  Gedamu, Lashitew³ 

[1] Départment de Chirurgie Orthopédique, Université de Montréal and Centre de Recherche Louis-Charles Simard, Hôpital Notre-Dame, 1560 Sherbrooke est Montréal, Montrál, Québec H2L 4M1, Canada;Départment of Médicine, Université de Montréal and Centre de Recherche Louis-Charles Simard, Hôpital Notre-Dame, 1560 Sherbrooke est, Montréal, Québec H2L 4MI, Canada;Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada

关键词: Chondrocyte;    Osteoarthritis;    Promoter;    Synoviocyte;    Transfection;   

DOI  :  10.1016/0014-5793(96)01037-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmids containing the reporter chloramphenicol acetyltransferase (CAT) gene attached to these promoters showed that SV40, stromelysin and MT promoters drove CAT expression with different strengths in primary bovine chondrocytes. The MTI-F and MT-IG gene promoters were also functional in human chondrocytes. The SV40, IL-1 inducible stromelysin-1 and MT-IG driven CAT activity was also detectable in human synoviocytes. Therefore, chondrocytes and synoviocytes have the trans-acting factors necessary for transcription from the respective promoters which may be conserved in bovine and human cells. These promoters could be useful for expressing potentially therapeutic anti-inflammatory and anti-erosive genes in arthritic joints.

【 授权许可】

Unknown