Excess formation of lysophosphatidic acid with age inhibits myristic acid‐induced superoxide anion generation in intact human neutrophils

FEBS Letters

Ito, Yoshikazu²  Lipschitz, David A.²  Ponnappan, Usha¹ 

[1] Department of Microbiology and Immunology, University of Arkansas for Medical Sciences and the Geriatric Research, Education and Clinical Center (GRECC), John L. McClellan Memorial Veterans Hospital, Little Rock, AR 72205, USA;Department of Medicine, University of Arkansas for Medical Sciences and the Geriatric Research, Education and Clinical Center (GRECC), John L. McClellan Memorial Veterans Hospital, 4300 West Seventh Street, Little Rock, AR 72205, USA

关键词: Aging;    Saturated fatty acid;    Oxidative burst;    Phospholipase;    Phosphatidic acid;    PLA2;    phospholipase A2;    PLC;    phospholipase C;    PLD;    phospholipase D;    PA;    phosphatidic acid;    LPA;    lysophosphatidic acid;    DG;    diacylglycerol;    IP3;    inositol trisphosphate;    TLC;    thin layer chromatography;    FMLP;    n-formyl-methionyl-leucyl-phenylalanine;    AA;    arachidonic acid;   

DOI  :  10.1016/0014-5793(96)00937-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A superoxide anion generation rate upon exposure to myristate of 1.93 ± 0.34 nmol/min/10⁶ cells in neutrophils from elderly human donors was significantly less than a value of 3.02 ± 0.48 nmol/min/10⁶ neutrophils from young donors. Myristate activation resulted in equal increases of AA in both the young and the old indicating no effect of aging on the PLA₂ pathway to response. By contrast, the PLD-induced generation of PA was significantly higher in the old than in the young. In addition, myristate induced a significant age-related enhancement in LPA generation, in the old but not in the young. The mass of LPA generated following activation was 3.5 nmol/ 2.5 × 10⁷cells/ml in the young while in the old it averaged 7.0 nmol/2.5 × 10⁷ cells/ml. The inhibitory effects of LPA may explain the age-related impaired ability to generate superoxide anion following activation by myristate.

【 授权许可】

Unknown