Expression of monocyte chemoattractant protein‐1 mRNA and protein in cultured human thyrocytes

FEBS Letters

Banba, Nobuyuki¹  Kasai, Kikuo¹  Shimoda, Shin-Ichi¹  Manaka, Kenichi²  Motohashi, Satoshi¹  Hattori, Yoshiyuki¹ 

[1]Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Mibu, Tochigi 321-02, Japan

[2]Center of Tissue Culture, Dokkyo University School of Medicine, Mibu, Tochigi 321-02, Japan

关键词: Thyrocyte;    Monocyte chemoattractant protein-1;    Interleukin-1;    Tumor necrosis factor-α;    Interferon-γ;    MCP;    monocyte chemoattractant protein;    IL;    interleukin;    TNF;    tumor necrosis factor;    IFN;    interferon;    TGF;    transforming growth factor;    RH;    recombinant human;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(96)00935-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monocytes as well as lymphocytes infiltrate in the stroma of thyroid tissue in autoimmune and destructive thyroiditis. Monocyte chemoattractant protein-1 (MCP-1) is a cytokine that attracts T-lymphocytes as well as monocytes. Using human thyrocytes in primary cultures, we show that expression of MCP-1 mRNA and protein is remarkably stimulated by both interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α), and also that interferon-γ (IFN-γ) by itself is a weak stimulant but has a synergistic activity with either IL-1 or TNF-α. The finding indicates that MCP-1 can be produced by thyrocytes themselves, suggesting a possible role of thyrocytes on accumulation of monocytes and T-lymphocytes to the tissue from the blood in autoimmune and destructive thyroiditis.

【 授权许可】

Unknown