cDNA cloning reveals a tissue specific expression of alternatively spliced transcripts of the ryanodine receptor type 3 (RyR3) calcium release channel

FEBS Letters

Sorrentino, Vincenzo¹  Marziali, Giovanna¹  Rossi, Daniela²  Giannini, Giuseppe¹  Charlesworth, Alexandra¹ 

[1] European Molecular Biology Laboratory, Heidelberg, Germany;DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy

关键词: Ryanodine receptor;    Alternative splicing;    RyR;    ryanodine receptor;    AS;    alternative splicing;    InsP3;    inositol 1;    4;    5-trisphosphate;    CaM;    calmodulin;   

DOI  :  10.1016/0014-5793(96)00944-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ryanodine receptors (RyRs) are a family of intracellular calcium release channels. Three cDNAs encoding different isoforms of RyR have been identified and cloned. We report the complete sequence of the mink RyR3 cDNA and the characterization of three alternative spliced regions. The first two splicing sites are represented by insertions of five and six amino acids, respectively. The third site is represented by a mutually exclusive splicing. The tissue distribution of the alternatively spliced transcripts revealed a ubiquitóus expression of splicing site I and a differential distribution of sites II and III, indicating that a further level of complexity in RyR3 expression may result from alternative splicings in this gene.

【 授权许可】

Unknown