Phenoxymethylpenicillin amidohydrolases from Penicillium chrysogenum

FEBS Letters

Abraham, E.P.¹  Whiteman, P.A.¹ 

[1] The Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK

关键词: Amidohydrolase;    Phenoxymethylpenicillin;    6-Aminopenicillanic acid;    Penicillium;    Penicillin biosynthesis;   

DOI  :  10.1016/0014-5793(96)00925-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A phenoxymethylpenicillin amidohydrolase which hydrolyses phenoxymethylpenicillin to 6-aminopenicillanic acid (6-APA) has been isolated from two species of Penicillium chrysogenum. The amidohydrolase had a molecular mass of approx. 42 kDa. Its activity with benzylpenicillin as substrate was only 1.5% of that with phenoxymethylpenicillin and it was inhibited by its products. No penicillin formation from 6-APA and phenoxyacetyl or phenylacetyl coenzyme A was observed. The enzyme is thus distinct from the phenylacetyl coenzyme A: 6-APA acyltransferase, which also has amidohydrolase activity and is involved in the final stages of the biosynthesis of penicillins.

【 授权许可】

Unknown