Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the molecule

FEBS Letters

Promé, J.-C.¹  Pueppke, Steven G.²  Krishnan, Hari B.²  Bec-Ferté, Marie-Pierre¹  Savagnac, Arlette¹ 

[1] Institut de Pharmacologie et de Biologie Structurale du Centre National de la Recherche Scientifique, 205 Route Norbonne, 31077 Toulouse Cedex, France;Department of Plant Pathology, University of Missouri, Columbia, MO 65211, USA

关键词: Lipochitooligosaccharides;    Nitrogen fixation;    Nod factors;    Nodulation;    Rhizobium fredii;    LCOs;    lipochitooligosaccharides;    LSIMS;    liquid secondary ionization mass spectrometry;    GC/MS;    gas chromatography/mass spectrometry;    MALDI;    matrix-assisted laser desorption/ionization;    CID;    collision-induced association;   

DOI  :  10.1016/0014-5793(96)00903-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flavonoid cues from the plant host cause symbiotic, nitrogen-fixing rhizobia to synthesize lipochitooligosaccharides (LCOs), which act as return signals to initiate the nodulation process. Rhizobium fredii strain USDA257 is known to produce four LCOs, all substituted with vaccenic acid (C_18:1). We show here that a mutant strain can replace vaccenic acid with a C_16:0 side chain, and that strain USDA191 synthesizes additional LCOs that differ from one another in the length and unsaturation of their fatty acyl substituents. USDA191 and 257DH4 both produce a novel LCO with glucose substituted for in the backbone of the molecule.

【 授权许可】

Unknown