Isolation and measurement of the endogenous cannabinoid receptor agonist, anandamide, in brain and peripheral tissues of human and rat

FEBS Letters

Nguyen, Duy N.²  Riggin, Ralph M.²  Palkovits, Miklós¹  Priller, Joseph¹  Axelrod, Julius¹  Richardson, John M.²  Koppel, Gary A.²  Felder, Christian C.¹  Becker, Gerald W.²  Paul, Steven M.²  Briley, Eileen M.¹  Nielsen, Amie¹ 

[1] The Laboratory of Cell Biology, Bldg. 36, Rm 3 A-15, National Institute of Mental Health, 36 Convent Drive MSC 4090, Bethesda, MD 20892-4090, USA;Lilly Research Laboratory, Lilly and Co., Indianapolis, IN 46285, USA

关键词: Anandamide;    Cannabis;    Cannabinoid receptor;    Marijuana;   

DOI  :  10.1016/0014-5793(96)00891-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anandamide (arachidonylethanolamide) is a novel lipid neurotransmitter first isolated from porcine brain which has been shown to be a functional agonist for the cannabinoid CB1 and CB2 receptors. Anandamide has never been isolated from human brain or peripheral tissues and its role in human physiology has not been examined. Anandamide was measured by LC/MS/MS and was found in human and rat hippocampus (and human parahippocampal cortex), striatum, and cerebellum, brain areas known to express high levels of CB1 cannabinoid receptors. Significant levels of anandamide were also found in the thalamus which expresses low levels of CB1 receptors. Anandamide was also found in human and rat spleen which expresses high levels of the CB2 cannabinoid receptor. Small amounts of anandamide were also detected in human heart and rat skin. Only trace quantities were detected in pooled human serum, plasma, and CSF. The distribution of anandamide in human brain and spleen supports its potential role as an endogenous agonist in central and peripheral tissues. The low levels found in serum, plasma, and CSF suggest that it is metabolized in tissues where it is synthesized, and that its action is probably not hormonal in nature.

【 授权许可】

Unknown