Localization of reactive tyrosine residues of baker's yeast transketolase

FEBS Letters

Baratova, Ljudmila¹  Kochetov, German¹  Viryasov, Mikhail¹  Kovina, Marina¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation

关键词: Transketolase;    Chemical modification;    Tyrosine;    Nitrotyrosine;    Proteolysis;    TrK;    transketolase;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(96)00836-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Baker's yeast transketolase inactivated by tetranitromethane was digested with Staphylococcus aureus V8 protease. Four peptides absorbing at 360 nm were isolated by reverse-phase HPLC and sequenced. The modified tyrosines were identified as Tyr-184, Tyr-210 and Tyr-370.

【 授权许可】

Unknown