Tumor necrosis factor α (TNFα) induces pro‐matrix metalloproteinase 9 production in human uterine cervical fibroblasts but interleukin 1 α antagonizes the inductive effect of TNFα

FEBS Letters

Mori, Yo¹  Sato, Takashi¹  Nagase, Hideaki²  Ito, Akira¹  Ogata, Yutaka² 

[1] Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-03, Japan;Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160-7421, USA

关键词: Matrix metalloproteinase 9;    Progelatinase B;    Tumor necrosis factor α;    Interleukin 1α;    Protein kinase C;    Human uterine cervical fibroblast;    MMP;    matrix metalloproteinase;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    IL-1;    interleukin 1;    TNFα;    tumor necrosis factor α;    MEM;    minimum essential medium;    PKC;    protein kinase C;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    H-7;    1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride;    HA1004;    N-(2-guanidinoethyl)-5-isoquinolinesulfonamide hydrochloride;   

DOI  :  10.1016/0014-5793(96)00808-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the regulation of precursor of matrix metalloproteinase 9 (proMMP-9)/progelatinase B production by tumor necrosis factor α (TNFα) and interleukin 1α (IL-1α) using human uterine cervical fibroblasts. TNFα, but not IL-1α, induces the production of proMMP-9 in the cervical cells. IL-1α, however, suppresses the TNFα-induced proMMP-9 production. 12-O-tetradecanoylphorbol 13-acetate (TPA) also stimulates the cervical cells to produce proMMP-9, and IL-1α synergistically enhances its production. TNFα-induced proMMP-9 production is not mediated by protein kinase C (PKC), whereas the effect of IL-1α is through PKC. By contrast, proMMP-3/prostromelysin 1 is up-regulated by TNFα or TPA in the presence of IL-1α, whose modulation is PKC-dependent. The suppressive effect of IL-1α on the TNFα-induced proMMP-9 production is a new biological effect of IL-1 on MMP production.

【 授权许可】

Unknown