Isolation and characterization of a cDNA encoding a sulfate transporter from Arabidopsis thaliana

FEBS Letters

Sasakura, Noriko¹  Takahashi, Hideki¹  Saito, Kazuki¹  Noji, Masaaki¹ 

[1] Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology in Research Center of Medicinal Resources, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263, Japan

关键词: Arabadopsis thaliana;    cDNA cloning;    Expressed sequence tag;    Sulfate transporter;    AST;    Arabidopsis thaliana sulfate transporter;    EST;    expressed sequence tag;    MSD;    membrane-spanning domain;   

DOI  :  10.1016/0014-5793(96)00787-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a sulfate transporter was isolated from Arabidopsis thaliana. The isolated clone contained an open reading frame encoding a polypeptide of 658 amino acids, exhibiting the highest similarity (62%) with the sequence of the low-affinity sulfate transporter of a tropical legume Stylosanthes hamata. Northern blot analysis indicated the constitutive accumulation of a 2.6 kb length transcript in leaves and roots of seedlings. We also propose that A. thaliana contains three sulfate transporter genes which are expressed as 3.0, 2.7 and 2.6 kb length transcripts, respectively, in an organ-specific manner.

【 授权许可】

Unknown