Phosphatidylcholine transfer protein from bovine liver contains highly unsaturated phosphatidylcholine species

FEBS Letters

Geijtenbeek, Teunis B.H.¹  Westerman, Jan¹  Wirtz, Karel W.A.¹  Heerma, Wigger² 

[1] Centre for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, P. O. Box 80.054, 3508 TB Utrecht, The Netherlands;Bijvoet Centre for Biomolecular Research Mass Spectrometry Group, Utrecht University, P.O. Box 80.083, 3508 TB Utrecht, The Netherlands

关键词: Phosphatidylcholine;    Mass spectrometry;    Phosphatidylcholine transfer protein;    Bovine liver;    Signal transduction;    PC-TP;    phosphatidylcholine transfer protein;    FABMS;    fast atom bombardment mass spectrometry;    CID;    collisional-induced dissociation;   

DOI  :  10.1016/0014-5793(96)00770-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phosphatidylcholine transfer protein (PC-TP) from bovine liver contains one molecule of non-covalently bound PC. In order to gain more insight into the physiological function of PC-TP, PC was extracted from bovine liver PC-TP and its molecular species composition identified by fast atom bombardment mass spectrometry. The prevailing molecular species were C18:0/C18:1-, C18:0/C18:2-, C18:OIC20:4-, C18:0120:5- and C18:OIC22:5-PC accounting for 85% of the PC species present. This molecular species composition is not representative for what is present in bovine liver where these species account for 43% of the total PC content [Montfoort et al. (1971) Biochim. Biophys. Acta 231, 335–342]. Another striking observation is that PC species carrying a palmitoyl chain at the sn-1 position are nearly absent, despite these species being abundantly present in bovine liver. This study suggests that PC-TP could play a role in the metabolism of highly unsaturated, stearoyl-containing PC species.

【 授权许可】

Unknown