The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges

FEBS Letters

Rollema, Harry S.²  Lagerwerf, Fija M.¹  van den Hooven, Henno W.²  Kuipers, Oscar P.²  Siezen, Roland J.²  Hilbers, Cornelis W.³  Heerma, Wigger¹  Haverkamp, Johan¹  Piard, Jean-Christophe² 

[1] Bijvoet Center for Biomolecular Research, Mass Spectrometry Group, Utrecht University, 3508 TB Utrecht, The Netherlands;Department of Biophysical Chemistry, NIZO (Netherlands Institute for Dairy Research), Kernhemseweg 2, 6718 ZB Ede, The Netherlands;NSR Center for Molecular Structure, Design and Synthesis, Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands

关键词: Bacteriocin;    Lanthionine-containing polypeptide;    Post-translational modification;    Cyanogen bromide cleavage;    NMR;    Mass spectrometry;    Ala∗ S;    3-methylalanyl moiety of (2S;    3S;    6R)-3-methyl-lanthionine;    AlaS;    d-alanyl moiety of meso-lanthionine;    sAla;    l-alanyl moiety of meso-lanthionine or of (2S;    3S;    6R)-3-methyllanthionine;    1/2/3D;    one-/two-/three-dimensional;    Dhb;    dehydrobutyrine;    DMSO;    dimethylsulfoxide;    DPC;    dodecylphosphocholine;    FAB;    fast-atom bombardment;    Hsl;    homoserine lactone;    MS;    mass spectrometry;    NOE;    nuclear Overhauser enhancement;    NOESY;    NOE spectroscopy;    SA-FF22;    streptococcin A-FF22;    TOCSY;    total correlated spectroscopy;   

DOI  :  10.1016/0014-5793(96)00771-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.

【 授权许可】

Unknown