Activation of platelet‐derived growth factor (PDGF) receptor dephosphorylation in intact Swiss 3T3 cells by elevators of intracellular Ca2+ and cAMP

FEBS Letters

Böhmer, Frank-D.¹  Böhmer, S.-Annette² 

[1] Max-Planck Society, Research Unit `Molecular Cell Biology' Medical Faculty, Friedrich-Schiller University, Drackendorfer Str. 1, D 07747 Jena, Germany;Institute of Biochemistry II, Medical Faculty, Friedrich-Schiller University, Löbderstr. 3, D 07743 Jena, Germany

关键词: Platelet-derived growth factor receptor;    Receptor dephosphorylation;    Protein tyrosine phosphatase;    Fibroblast (Swiss 3T3);    DMEM;    Dulbecco's modified Eagle medium;    EGF;    epidermal growthh;    PDGF;    platelet-derived growth factor;    PKC;    protein kinase;    PTPase;    protein tyrosine phosphate;   

DOI  :  10.1016/0014-5793(96)00740-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the modulation of platelet-derived growth factor (PDGF) receptor dephosphorylation in Swiss 3T3 cells using a novel assay permitting monitoring of receptor dephosphorylation in intact cells. PDGF treatment of the cells reduced the receptor dephospphorylation rate to 41%, the elevators of intracellular Ca²⁺, A23187 and thapsigargin increasing it to 227 and 138%, respectively. The cAMP elevators forskolin and isobutylmethylxanthine also accelerated PDGF receptor dephosphorylation. The involvement of Ca²⁺- and cAMP-dependent protein kinases in the regulation of PDGF receptor dephosphorylation is suggested.

【 授权许可】

Unknown