Conformational change in DNA induced by cationic bilayer membranes

FEBS Letters

Akao, Tetsuyuki³  Ihara, Hirotaka¹  Fukumoto, Takanori⁴  Ito, Akio² 

[1] Department of Applied Chemistry, Faculty of Engineering, Kumamoto University, Kumamoto 860, Japan;Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka 812-81, Japan;Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Aikawa, Kurume, Fukuoka 830, Japan;Chemical and Textile Industry Research Institute, Fukuoka Industrial Technology Center, Kamikoga, Chikushino, Fukuoka 818, Japan

关键词: Bilayer membrane;    Cationic lipid;    DNA conformation;    Ethidium bromide;    CTAB;    cetyltrimethylammonium bromide;    TMAB;    trimethylammonium bromide;   

DOI  :  10.1016/0014-5793(96)00736-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of synthetic cationic lipids on the structure of DNA was studied. The fluorescence enhancement of ethidium bromide on intercalation into DNA was suppressed by the addition of bilayer-forming lipids, but not by micellar ones. Results on the fluorescence depolarization index suggest that ethidium bromide is not released from DNA by lipids intercalated into DNA. CD spectra of the DNA-lipid complexes revealed that the structure of DNA was changed only by bilayer-forming lipids at temperatures lower than their T _c values. Thus, the conformation of DNA is forced to change by cationic lipids forming the rigid bilayer membrane so that ethidium bromide fluorescence might be reduced, and the conformation can be controlled by selection of the appropriate lipid and temperature.

【 授权许可】

Unknown