Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: implications in receptor solubilization

FEBS Letters

Harikumar, K.G.¹  Chattopadhyay, Amitabha¹ 

[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

关键词: Critical micelle concentration;    CHAPS;    3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate;    Ionic strength;    5-HT1A receptor;    Solubilization;    Zwitterionic detergent;    ABC;    bicinchoninic acid;    CHAPS;    3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate;    CMC;    critical micelle concentration;    DPH;    1-6-diphenyl-1;    3;    5-hexatriene;    EDTA;    ethylene-diaminetetraacetic acid;    EGTA;    ethylene glycol bis(β-aminoethyl-ether)-N;    N;    N′;    N′-tetraacetic acid 5-HT;    5-hydroxytryptamine;    OH-DPAT;    8-hydroxy-2-(di-[propylamino)tetralin;    PEG;    polyethylene glycol;    PMSF;    phenylmethylsulfonyl fluoride;    SDS;    sodium dodecyl sulfate;    THF;    tetrahydrofuran;    TRIS;    tris-(hydroxymethyl)amino-methane;   

DOI  :  10.1016/0014-5793(96)00733-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT_1A) from bovine brain hippocampal crude (native) membrane by CHAPS at premicellar concentration under high salt conditions.

【 授权许可】

Unknown