Action of bovine serum albumin on cytochrome c oxidase activity and proton pumping: a role for fatty acids in enzyme function?

FEBS Letters

Perin, Ivano¹  Sharpe, Martyn¹  Nicholls, Peter¹ 

[1] Department of Biological Sciences,, Brock University, St. Catharines, Ont. L2S 3A1, Canada

关键词: Cytochrome c oxidase;    Fatty acids;    Palmitate;    Oleate;    Bovine serum albumin;    Spectral change;    Proteoliposomes;    Respiratory control;    Proton pumping;   

DOI  :  10.1016/0014-5793(96)00721-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine serum albumin (BSA) at micromolar concentrations causes a red shift of the Soret band of bovine cytochrome c oxidase with a slow biphasic time course. It also inhibits the turnover of detergent-isolated enzyme in a similarly slow manner; the progress of this inhibition is halted by palmitate and other fatty acids. The inhibitory bovine serum albumin effect may involve fatty acid depletion from the enzyme. Respiration by cytochrome c oxidase vesicles (proteoliposomes) in the presence of ionophores (uncontrolled) shows only a small inhibition by BSA but preincubation of such vesicles with BSA induces a loss of proton pumping activity. After incubation of BSA-depleted proteoliposomes in the presence of reductant with combinations of fatty acids, pumping activity can be fully restored, suggesting a supportive or even essential role of endogenous fatty acids in H⁺ translocation by this membranous enzyme.

【 授权许可】

Unknown