Fructose 2,6‐bisphosphate induces irreversible transitions in cell‐free extracts of rat liver

FEBS Letters

Schellenberger, Wolfgang¹  Frenzel, Jochen¹  Eschrich, Klaus¹ 

[1] Institute of Biochemistry, University of Leipzig, School of Medicine, Liebigstraße 16, D-04103 Leipzig, Germany

关键词: Bistability;    Glycolysis;    Hysteresis;    Irreversible transition;    Nonlinear dynamics;    Phosphofructokinase/fructose-1;    6-bisphosphatase cycle;   

DOI  :  10.1016/0014-5793(96)00664-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of fructose 2,6-bisphosphate on the dynamics of the 6-phosphofructo-1-kinase/fructose-1,6-bisphos-phatase cycle is investigated in a cell-free extract of rat liver under steady-state conditions. Bistability emerges on the basis of the reciprocal allosteric modulation of 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase. Under conditions of bistability fructose 2,6-bisphosphate may cause transitions between alternative steady states. However, in contrast to what is frequently observed in bistable systems, within a broad range of experimental conditions these transitions proceed irreversibly from states with high ATP to states characterized by low ATP.

【 授权许可】

Unknown