The phosphorylation state of MAP‐kinases modulates the cytotoxic response of smooth muscle cells to hydrogen peroxide

FEBS Letters

Boscoboinik, Daniel³  Azzi, Angelo³  Cantoni, Orazio¹  Fiorani, Mara²  Stäuble, Barbara³ 

[1] Istituto di Chimica Biologica, Università degli Studi di Urbino, Urbino, Italy;Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Urbino, Italy;Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstrasse 28, 3012 Bern, Switzerland

关键词: Hydrogen peroxide;    Cytotoxicity;    Mitogen-activated protein kinase;    Protein-tyrosine kinase;    Protein kinase C;    MAP-kinase;    mitogen-activated protein kinase;    PTK;    protein-tyrosine kinase;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;    LDH;    lactate dehydrogenase;    DMEM;    Dulbecco's modified Eagle medium;    FBS;    foetal bovine serum;    NaCl/Pi;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(96)00605-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Micromolar concentrations of hydrogen peroxide induced the phosphorylation of mitogen-activated protein (MAP) kinases and a lethal response in growth-arrested smooth muscle cells (A7r5). The H₂O₂-induced phosphorylation of MAP-kinases was markedly lower in the presence of protein tyrosine kinase (PTK) inhibitors or in protein kinase C (PKC) down-regulated cells. Similarly, the toxicity of H₂O₂ was diminished by concomitant addition of either PKC or PTK inhibitors and was also lower in PKC down-regulated cells. These results are consistent with the possibility that phosphorylation of MAP-kinases is a critical event in the toxic response of cultured smooth muscle cells to H₂O₂.

【 授权许可】

Unknown