FEBS Letters | |
The phosphorylation state of MAP‐kinases modulates the cytotoxic response of smooth muscle cells to hydrogen peroxide | |
Boscoboinik, Daniel3  Azzi, Angelo3  Cantoni, Orazio1  Fiorani, Mara2  Stäuble, Barbara3  | |
[1] Istituto di Chimica Biologica, Università degli Studi di Urbino, Urbino, Italy;Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Urbino, Italy;Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstrasse 28, 3012 Bern, Switzerland | |
关键词: Hydrogen peroxide; Cytotoxicity; Mitogen-activated protein kinase; Protein-tyrosine kinase; Protein kinase C; MAP-kinase; mitogen-activated protein kinase; PTK; protein-tyrosine kinase; PKC; protein kinase C; PMA; phorbol 12-myristate 13-acetate; LDH; lactate dehydrogenase; DMEM; Dulbecco's modified Eagle medium; FBS; foetal bovine serum; NaCl/Pi; phosphate-buffered saline; | |
DOI : 10.1016/0014-5793(96)00605-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Micromolar concentrations of hydrogen peroxide induced the phosphorylation of mitogen-activated protein (MAP) kinases and a lethal response in growth-arrested smooth muscle cells (A7r5). The H2O2-induced phosphorylation of MAP-kinases was markedly lower in the presence of protein tyrosine kinase (PTK) inhibitors or in protein kinase C (PKC) down-regulated cells. Similarly, the toxicity of H2O2 was diminished by concomitant addition of either PKC or PTK inhibitors and was also lower in PKC down-regulated cells. These results are consistent with the possibility that phosphorylation of MAP-kinases is a critical event in the toxic response of cultured smooth muscle cells to H2O2.
【 授权许可】
Unknown
【 预 览 】
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