αIIbβ3‐integrin mediated adhesion of human platelets to a fibrinogen matrix triggers phospholipase C activation and phosphatidylinositol 3′,4′‐bisphosphate accumulation

FEBS Letters

Payrastre, Bernard¹  Racaud-Sultan, Claire¹  Kieffer, Nelly²  Chap, Hugues¹  Gironcel, Daisy¹  Horicot, Martine¹  Borchert, Gudrun¹  Breton, Monique¹ 

[1] INSERM U 326, Phospholipides Membranaires, Signalisation Cellulaire et Lipoprotéines, Hôpital Purpan, 31059 Toulouse Cedex, France;Laboratoire Franco-Luxembourgeois de Recherche Biomédicale, 162A avenue de la Faïencerie, L-1511 Luxembourg, Luxembourg

关键词: αIIbβ3-Integrin;    Platelet adhesion;    Phospholipase C;    Phosphatidylinositol 3-kinase;    ADP;    PLC;    phospholipase C;    PLD;    phospholipase D;    PtdIns;    phosphatidylinositol;    PtdIns(3)P;    phosphatidylinositol 3′-phosphate;    PtdIns(4)P;    phosphatidylinositol 4′-phosphate;    PtdIns(3;    4)P2;    phosphatidylinositol 3′;    4′-bisphosphate;    PtdIns(4;    5)P2;    phosphatidylinositol 4′;    5′-bisphosphate;    PtdIns(3;    4;    5)P3;    phosphatidylinositol 3′;    4′;    5′-trisphosphate;    PtdIns 3-kinase;    phosphatidylinositol 3-kinase;    PtdOH;    phosphatidic acid;    TXA2;    thromboxane A2;   

DOI  :  10.1016/0014-5793(96)00595-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study focused on the variations in phosphoinositide metabolism depending upon α_IIbβ₃-integrin/fibrinogen interaction without previous activation of platelet agonist receptors. We found that adhesion of resting human platelets to immobilized fibrinogen stimulates phosphatidic acid production and a concomitant decrease in phosphatidylinositol 4′,5′-bisphosphate. These results, and the absence of a transphosphatidylation reaction, argue in favor of the activation of a phospholipase C. Moreover, we observed the accumulation of phosphatidylinositol 3′,4′-bisphosphate in adherent platelets as a consequence of the activation of a phosphatidylinositol 3-kinase. This effect was inhibited by ADP scavengers. Our results demonstrate that in adherent platelets, whereas phosphatidylinositol 3-kinase activation is controlled by both α_IIbβ-integrin engagement and released ADP, phospholipase C stimulation is triggered only by α_IIbβ-integrin/fibrinogen interaction.

【 授权许可】

Unknown