Cloning, sequencing and functional assignment of the chlorophyll biosynthesis gene, chlP, of Synechocystis sp. PCC 6803

FEBS Letters

Hunter, C.Neil¹  Jensen, Poul E.¹  Addlesee, Hugh A.¹  Gibson, Lucien C.D.¹ 

[1]Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2 UH, UK

关键词: Photosynthesis;    (Bacterio)chlorophyll;    Synechocystis;    Rhodobacter;    Hydrogenase;    Phytol;    bchl;    bacteriochlorophyll;    chl;    chlorophyll;    GG;    geranylgeraniol;    GGPP;    geranylgeranyl pyrophosphate;    P;    phytol;    PPP;    phytyl pyrophosphate;    DHGG;    dihydro-GG;    THGG;    tetrahydro-GG;    LH1;    light-harvesting complex 1;    LH2;    light-harvesting complex 2;    HPLC;    high-pressure liquid chromatography;    ORF;    open reading frame;   

DOI  :  10.1016/0014-5793(96)00549-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene from the cyanobacterium Synechocystis sp. PCC 6803 has been cloned and sequenced, and subsequently used to partially complement a bchP mutant of the purple photosynthetic bacterium Rhodobacter sphaeroides. This mutant is blocked in the terminal hydrogenation steps of bchla biosynthesis and possesses only bchl esterified with geranylgeraniol. It also has a reduced cellular level of the light-harvesting LH2 complex, and the 850 nm absorbance maximum of LH2 is red-shifted by approximately 6 nm. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of bchlaGG detectable, but the level of LH2 is increased and the red-shift reversed by several nm. We conclude that this gene, which we term chlP, encodes the enzyme catalysing the stepwise hydrogenation of geranylgeraniol to phytol during chla biosynthesis.

【 授权许可】

Unknown