Leukotriene A4, and not leukotriene B4, is the main 5‐lipoxygenase metabolite released by bovine leukocytes

FEBS Letters

Testa, Tullio¹  Folco, Giancarlo²  Sala, Angelo² 

[1] Bassini Hospital, Via M. Gorki 50, 20092 Cinisello B., Italy;Center for Cardiopulmonary Pharmacology, Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milano, Italy

关键词: Transcellular metabolism;    Leukotriene A4;    Leukotriene C4 synthase;    (Bovine polymorphonuclear leukocyte);    (Bovine platelet);    LTA4;    leukotriene A4;    LTB4;    leukotriene B4;    LTC4;    leukotriene C4;    PMNL;    polymorphonuclear leukocytes;    5-LO;    5-lipoxygenase;    PBS=;    PBS without Ca2+;    Mg2+;    PRP;    platelet-rich plasma;    Δ 6-trans-LTB4 isomers;    5(S) 12(R)-dihydroxy-6;    8;    10-trans-14-cis-eicosatetraenoic acid;    5(S);    12(S)-dihydroxy-6;    8;    10-trans-14-cis-eicosatetraenoic acid;    5;    12-diHETE;    5(S);    12(S)-dihydroxy-6;    10-trans-8;    14-cis-eicosatetraenoic acid;    5;    6-diHETE isomers;    5(S);    6(S)-dihydroxy-7;    9-trans-11;    14-cis-eicosatetraenoic acid;    5(S);    6(R)-dihydroxy-7;    9-trans-11;    14-cis-eicosatetraenoic acid;   

DOI  :  10.1016/0014-5793(96)00539-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The production of leukotriene A₄ (LTA₄)-derived metabolites, analysed by RP-HPLC, was studied in purified bovine polymorphonuclear leukocyte (PMNL) preparations and in PMNL-platelet coincubations after challenge with the calcium ionophore A23187. The results obtained show that in bovine PMNL LTB₄ represents the main LTA₄ metabolite. When washed platelets were added to PMNL, LTC₄ was the main enzymatic metabolite observed, indicating a substantial transfer of PMNL-derived LTA₄ to platelets. The synthesis of LTC₄ was accompanied by a significant decrease in LTB₄, suggesting that a quota of the LTB₄ synthesized in PMNL preparations is the result of transcellular metabolism of released LTA₄ by neighbouring PMNL. Reduction of PMNL-PMNL interactions through dilution of cell incubates allowed us to estimate that most of the leukotriene A₄ synthesized by PMNL is indeed released from the cell. LTA₄, and not LTB₄, represents the main 5-lipoxygenase metabolite released by bovine PMNL.

【 授权许可】

Unknown