Heat‐labile uracil‐DNA glycosylase: purification and characterization

FEBS Letters

Sobek, H.¹  Kaluza, K.¹  Schmidt, M.¹  Frey, B.¹ 

[1] Boehringer Mannheim GmbH, Nonnenwaldstr. 2, 82377 Penzberg, Germany

关键词: Uracil-DNA glycosylase;    Carryover prevention;    PCR;    Amplification;    UNG;    uracil-DNA glycosylase;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(96)00444-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40°C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.

【 授权许可】

Unknown